Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology.

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1 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Perhitungan Jumlah Mikrobia Nur Hidayat Kuliah Bio-Industri

2 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Pembelahan sel tunas Spora Fragmentasi hifa Reproduksi

3 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Pembelahan Sel Figure 6.11

4 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.12b

5 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Jika 100 sel ditumbuhkan selama 5 jam menghasilkan 1.720.320 sel maka:

6 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.13

7 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.14

8 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Jumlah koloni: Dilakukan dengan pengenceran sampel Perhitungan mikrobia berdasar jumlah koloni Figure 6.15, top portion

9 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Inokulasi cawan petri dari seri pengenceran Metode Plating Figure 6.16

10 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Setelah inkubasi,hitung koloni pada cewan yang memiliki jumlah 25-250 koloni (CFU) Penghitungan Koloni Figure 6.15

11 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Syarat-syarat perhitungan Jumlah koloni 25 – 250. Jika ada dua ulangan dirata-rata, perbandingan dua pengenceran berurutan jika lebih besar atau sama dengan 2 gunakan pengenceran sebelumnya, jika kurang dari 2 dirata-rata. Contoh: PengenceranPetri 1Petri 2 10 -2 228240 10 -3 2823 Maka: (280+230)/(228+240) = 510/468 < 2 maka Jumlah sel = (280+230+228+240)/4 = 244,5 X10 2 = 2,5 X10 4

12 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Penggunaan Haemacytometer Figure 6.19

13 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Perhitungan secara langsung dengan mikroskop Menggunakan haemacytometer atau Petroff Houser Counter. Misal Diket Luas Kotak pada haemacytometer = 4 X 10 -2 mm 2 Tinggi = 2 X 10 -2 mm Maka volume daerah kotak = 8 X 10 -4 mm 3 = 8 X 10 -7 ml Jumlah sel dihitung 14 Maka jumlah mikrobia

14 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Penghitungan dengan cara tidak langsung Aktivitas metabolit Berat kering

15 Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sampai jumpa