STREPTOCOCCI.

Presentasi berjudul: "STREPTOCOCCI."— Transcript presentasi:

1 STREPTOCOCCI

2 (staphylococci are catalase positive)
Streptococci facultative anaerobe Gram-positive chains or pairs catalase negative (staphylococci are catalase positive)

3 Streptococcus in chains

4 Streptococcus pneumoniae (diplococcus). Fluorescent stain

5 Streptococci Lancefield groups Satu atau lebih spesies per group antigen permukaan

6 groupable streptococci
A, B and D most important C, G, F rare

7 Non-groupable S. pneumoniae viridans streptococci pneumonia
e.g. S. mutans dental caries

8 hemolysis reaction - sheep blood agar
α (alpha) partial hemolysis green color β (beta) complete clearing A and B γ (gamma) no lysis

9 Hemolysis Groups A an B β Group D α or γ S. pneumoniae and viridans γ

10 hemolysis reaction + one characteristic
presumptive identification

11 Group A streptococcus (S. pyogenes)

12 Group A streptococcal menginfeksi semua umur
Insiden tertinggi pd usia 5-15 thn

13 S. pyogenes -suppurative
non-invasive pharyngitis Infeksi kulit, impetigo invasive bacteremia toxic shock-like syndrome "flesh eating" bacteria pyrogenic toxin

14 Pyrogenic toxin superantigen T cell mitogen Mengaktifkan sistem imun

15 Scarlet fever rash erythrogenic toxin

16 non-suppurative rheumatic fever inflammatory disease life threatening
Sekuel kronik demam jantung sendi rheumatic tidak sama dengan rheumatoid arthritis

17 Rheumatic fever -etiology
M protein cross-reacts heart myosin autoimun Antigen dinding sel sedikit dicerna in vivo bertahan lama

18 Rheumatic fever penicillin menyembuhkan pharyngitis
Menurunkan resiko carditis

19 Acute glomerulonephritis
immune complex disease of kidney

20 Major pathogenesis factors
lipoteichoic acid/F protein fimbriae binds to epithelial cells M protein anti-phagocytic

21 M protein major target strain variation re-infection Imunitas alami
antigenicity re-infection Terjadi dengan strain yg berbeda

22 Capsules Anti-phagocytic mucoid strains

23 Isolation and identification
β hemolytic colonies bacitracin menghambat pertumbuhan β hemolytic colonies group A antigen

24 β hemolysis hemolysin O hemolysin S Sensitif oksigen
Non sensitive oksigen

25 Post-infectious diagnosis (serology)
Antibodi thd streptolysin O Penting! Bila menetap sekuel kronik terjadi

26 Serotyping M T R

27 Group B streptococcus neonatal meningitis septicemia transmission
vaginal flora

28 Group B streptococcus - identification
β hemolysis hippurate hydrolysis CAMP reaction meningkatkan β hemolisis S. aureus

29 Group D streptococcus Tumbuh pada bile esculin agar precipitasi hitam
6.5% salin Tumbuh enterococci Tidak tumbuh non-enterococci

30 Enterococci Hubungannya agak jauh dr streptococci lain
genus Enterococcus Flora usus urinary tract infection Kontaminasi dr anus opportunistic infections khususnya endocarditis Paling sering E. (S.) faecalis

31 Enterococci resisten thd banyak antibiotik termasuk vancomycin
ujung D-ala diganti dengan D-lactat

32 Minute colony streptococci
Various groups (e.g. group A) berbeda scr genetik koloni yg besar (e.g. S. pyogenes) tdk ada demam rheuma Koloni besar Koloni kecil

33 Viridans streptococci
diverse species oral Karies gigi α hemolytic dan negatif thd tes lain non-groupable. termasuk S. mutans endocarditis cabut gigi