LIPOSOM Agustina Setiawati.

Presentasi berjudul: "LIPOSOM Agustina Setiawati."— Transcript presentasi:

1 LIPOSOM Agustina Setiawati

2 DEFINISI Liposom adalah dimensi koloid berupa vesikel yang mempunyai struktur fosfolipid bilayer dan mempunyai muatan permukaan tertentu (positif, negatif atau netral) Hydrophilic Hydrophobic

3 STRUKTUR UMUM

4 Classes of Liposomes Conventional Cationic Immuno

5 Liposome kationik Liposome anionik Liposome non-ionik Liposome Zwitterionik Which one can form complexes with DNA?

6 Kationik Liposome Cationik liposome forms complex with DNA (negative charge) Net charge of the complexes is positive so it can be interact to cell membrane

7 Mekanisme

8 Mekanisme…

9 Anionik Liposom Can’t it deliver DNA? Yess Mekanisme?
DNA tidak bisa membentuk kompleks dgn liposom anionik (muatan negatif-negatif) Supaya bs, DNA dibuat kompleks lebih dahulu dgn kation (Ca2+)

10 Liposome dpt digunakan sbg vektor terapi gen scr in vivo atau ex vivo?

11 1995 liposome Cystic Fibrosis Trials using liposome-mediated CFTR gene transfer began in 1995. Non-viral vectors have the potential to avoid some of the critical problems observed with viral vectors, such as the immune response, limited packaging capacity, and random integration5 . Liposomes may be mildly effective, but their activity does not last. For this approach to work, researchers need to figure out how to improve delivery, make the effects more permanent and reduce the adverse side effects. To date, only cationic liposome-based systems have been tested in clinical trials in cystic fibrosis subjects6.

12 Kelebihan Tidak menimbulkan respon imunologis
Ukuran DNA yang dapat dibawa beragam Modifikasi lebih fleksibel

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14 THANK YOU