PENYEDIAAN ANTISERUM POLIKLONAL TERHADAP COAT PROTEIN (CP) DARI TOMATO CHLOROSIS VIRUS (ToCV) YANG DIEKSPRESIKAN PADA Escherichia coli Gede Suastika &

Presentasi berjudul: "PENYEDIAAN ANTISERUM POLIKLONAL TERHADAP COAT PROTEIN (CP) DARI TOMATO CHLOROSIS VIRUS (ToCV) YANG DIEKSPRESIKAN PADA Escherichia coli Gede Suastika &"— Transcript presentasi:

1 PENYEDIAAN ANTISERUM POLIKLONAL TERHADAP COAT PROTEIN (CP) DARI TOMATO CHLOROSIS VIRUS (ToCV) YANG DIEKSPRESIKAN PADA Escherichia coli Gede Suastika & Giyanto Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor

2 OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA
Since 2006, outbreaks of yellowing disease on tomato (Lycopersicon esculentum) crops have occurred in several regions of Indonesia. Especially in upland of Garut and Cipanas [West Java], Magelang [Central Java], Bedugul [Bali] and Sembalun [Lombok].

3 OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA
Appearance of yellowing diseases affected tomato plants found in Garut [West Java], Magelang [Central Java], Bedugul [Bali] and Sembalun [Lombok].

4 OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA
The disease was characterized by interveinal yellowing that developed initially on lower leaves and then progressed to the upper part of the plant. No obvious symptoms are produced on fruit, but significant yield reduction occurs.

5 DETECTION OF THE VIRUSES BY USING RT-PCR
The yellowing disease symptoms resembled to those described in USA, Spain and others counties for the infections caused by Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) Both viruses are belong to Family Closteroviridae; Genus Crinivirus Crinivirus has flexuous, filamentous particles [12 x nm]; a bipartite, single-stranded RNA genome [RNA 1 and RNA 2 are 7.8 and 7.4 kb, respectively] (Duffus et al. 1996)