Penggunaan CSSLs untuk pencarian lokasi gen dalam kromosom

Presentasi berjudul: "Penggunaan CSSLs untuk pencarian lokasi gen dalam kromosom"— Transcript presentasi:

1 Penggunaan CSSLs untuk pencarian lokasi gen dalam kromosom
Bogor, 21 Februari 2013

2 ? ? Apakah CSSLs itu? ?

3 Strategi dasar pemetaan QTL (lokus sifat kuantitatif)
Fenotipe: kerapatan trikoma daun Populasi: RILs (recombinant inbred lines) Mauricio, Nature Reviews Genetics, 2001

4 Metode pemetaan QTL (lokus sifat kuantitatif)
Fenotipe: kerapatan trikoma daun Populasi: F2 Mauricio, Nature Reviews Genetics, 2001

5 Pemetaan awal mencari lokasi Istana Bogor

6 Pemetaan halus mencari lokasi Istana Bogor

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8 Tahap-tahap berurutan dalam kloning gen-gen QTL
Semua tahap ini makan banyak waktu dan tenaga Ashikari & Matsuoka, Trends in Plant Science, 2006

9 Produksi Introgression Lines (ILs) atau CSSLs
Ashikari & Matsuoka, Trends in Plant Science, 2006

10 Chromosome Segment Substitution Lines (CSSLs)
Ebitani et al., Breeding Science , 2005

11 Pemetaan QTL menggunakan Introgression Lines (ILs) atau CSSLs
Paran & Zamir, Trends in Genetics, 2003

12 Introgression lines of L
Introgression lines of L. pennellii in cultivated tomato for QTL mapping FM = fruit mass Left: a fruit from the wild tomato species L. pimpinellifolium Right: a fruit from L. esculentum cv. Giant Red, bred to produce extremely large tomatoes Eshed & Zamir, Genetics, 1995

13 Figure 2 High-resolution mapping of the fw2.2 QTL.
A Frary et al. Science 2000;289:85-88 Published by AAAS

14 Phenotypic effect of the fw2.2 transgene in L. esculentum
pCLD04541 Fruit from R1 progeny of fw107 segregating for the presence (+) or absence (-) of cos50 The exact molecular function of fw2.2 is not known, although it shares homology with the human RAS oncogenes in protein structure and contains two predicted transmembrane-spanning domains. fw2.2 acts as a negative regulator of cell division in the fruit, perhaps via some role in cell-to-cell communication. A Frary et al. Science 2000;289:85-88 Published by AAAS

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16 Positions of QTLs for field resistance to rice blast in Japanese upland rice
qBR4-2 146 F4 lines (NipponbarexOwarihatamochi) 111 RFLP markers pi21, a recessive gene encoding a proline-rich protein (Fukuoka et al., Science, 2009) Fukuoka & Okuno, TAG, 2001

17 Pengembangan bahan tanaman Sub-CSSLs
Fukuoka et al., TAG, 2012

18 Graphical genotypes dari Sub-CSSLs sekitar qBR4-2
Fukuoka et al., TAG, 2012

19 Graphical genotypes dari Sub-CSSLs sekitar qBR4-2a dan qBR4-2b
Fukuoka et al., TAG, 2012

20 Pyramiding qBR4-2a, qBR4-2b, dan qBR4-2b
Fukuoka et al., TAG, 2012

21 Perbandingan sekuen qBR4-2a antara Nipponbare dan Owarihatamochi
Fukuoka et al., TAG, 2012

22 Peranan protein NBS-LRR dalam ketahanan tanaman terhadap patogen
Spoel & Dong, Nat Rev Immunology, 2012

23 Analisis filogenetik qBR4-2a dan 30 gen R yang lain
Fukuoka et al., TAG, 2012

24 Terima kasih Terima kasih