MATERI 7 PERKEMBANGAN KONSEPTUS MK. Ilmu Reproduksi LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB SUB POKOK BAHASAN Proses pembelahan sel zygote Differensiasi sel embrio dan perkembangan embrio awal Proses implantasi Faktor-faktor yang mempengaruhi perjalanan embrio hingga terjadinya implantasi LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Ovulation Only fertilized ova are transported into the the uterine horn of the mare Oviduct Unfertilized Ova Ovary Oviductal Transport in the Mare Uterine Horn Blastocyst PGE Release LABORATORIUM REPRODUKSI TERNAK FAPET UB
Ovulasi dan Fertilisasi LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Early Embryonic Development Location Day Development Ampullary- 0-2 One cell Isthmic Junction Two Polar Bodies All cell divisions are mitotic Smaller cell size with each cleavage division Separate Blastomere’s for twins Ampullary- Isthmic Junction 1-3 Two cell Isthmus 2-3 Four cell Isthmus 3-5 Eight cell Enters uterus - Even if not fertilized Except Mare - Unfertilized remain in oviduct Uterus 4-5 Sixteen cell Fuse two morula together for a chimera Uterus 5-8 Morula LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Day Development Compact Morula form tight cell contacts so that Blastocyst formation is possible 6-7 Tight Morula 7-8 Early Blastocyst Fills with fluid in center - Cells have established polarity 7-9 Blastocyst Blastocoel Inner Cell Mass - Forms Embryo Trophoblast - Forms Placenta If split ICM get twins 8-10 Expanded Blastocyst Contraction and expansion of blastocyst as well as enzymes are involved with zona removal 9-11 Hatching Blastocyst Picture reproduced with permission of Academic Press In: Physiology and Technology of Reproduction Picture courtesy of Dr. J.E. Flechon LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Unpolarized Morula Polarized Morula Inner Cell Mass Blastocoel Blastocyst Trophectoderm Blastocoel LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Embrio Hatching Terjadi Implantasi (Ternak Bunting) LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Embryo sapi LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Blastocyst - Nuclei Inner Cell Mass LABORATORIUM REPRODUKSI TERNAK FAPET UB Photomicrograph courtesy of Dr. Daniel Pomp
LABORATORIUM REPRODUKSI TERNAK FAPET UB Time comparisons from ovulation during cleavage for different farm species 1 cell 8 Cell Blastocyst Enter Uterus Length of Species (hours) (Days) Gestation Cattle 24 3 8 3.5 281 Horse 24 3 6 5 337 Sheep 24 2.5 7 3 148 Swine 14-16 2 6 2 114 Note - Late entry in mare LABORATORIUM REPRODUKSI TERNAK FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB Differentiation o f Conceptus Cell Types Inner Cell Mass 1 2 3 Three Layers Form Day 14 Expanded Blastocyst LABORATORIUM REPRODUKSI TERNAK FAPET UB