STUDY ON INSECT TRANSMISSION OF THE VIRUSES Ten Bemisia tabaci or Trialeurodes vaporariorum allowed a 24-h acquisition feeding on ToCV- and TICV- double infected plants and then allowed a 24-h inoculation feeding on tomato plants. Whitefly species Number of inoculated plants Percentage of infected plants ToCV TICV ToCV & TICV B. tabaci 10 80.0 0.0 T. vaporariorum 60.0 30.0 10.0
STUDY ON INSECT TRANSMISSION OF THE VIRUSES Single Bemisia tabaci allowed a 24-h acquisition feeding on diseased plants are capable of transmitting ToCV. Virus Number of B. tabaci/plant Percentage of infected plants Incubation period (hr) ToCV 1 13.3b 10-12 3 20.0b 18-19 5 33.3b 19-20 7 66.7a 19-21 10 80.0a 14-18 Transmission rates established by 1, 3, 5, 7, and 10 whiteflies per plant were: 13.3, 20.0, 33.3, 66.7, and 80.0%, respectively.
STUDY ON INSECT TRANSMISSION OF THE VIRUSES Single Trialeurodes vaporariorum allowed a 24-h acquisition feeding on diseased plants are capable of transmitting ToCV. Virus Number of T. vaporariorum/plant Percentage of infected plant Incubation period (hr) ToCV 1 6.7c 12 3 26.7b 14 5 33.3b 16 7 60.0a 15-17 10 70.0a 11-13 Transmission rates established by 1, 3, 5, 7, and 10 whiteflies per plant were: 6.7, 26.7, 33.3, 60.0, and 70.0%, respectively.
STUDY ON INSECT TRANSMISSION OF THE VIRUSES Single Trialeurodes vaporariorum allowed a 24-h acquisition feeding on diseased plants are capable of transmitting TICV. Virus Number of T. vaporariorum/plant Percentage of infected plant Incubation period (hr) TICV 1 13.3b 11 3 26.7ab 21-22 5 40.0ab 16-17 7 60.0a 18-19 10 100.0a 9-26 Transmission rates established by 1, 3, 5, 7, and 10 whiteflies per plant were: 13.3, 26.7, 40.0, 60.0, and 100.0%, respectively.
KENDALA DALAM DETEKSI INFEKSI VIRUS PENYEBAB KLOROSIS Gejala khlorosis akibat infeksi ToCV mirip dengan gejala-gejala oleh penyebab lain: (1) ketidak-normalan fungsi fisiologi; (2) masalah unsur hara; (3) keracunan bahan kimia dari pestisida Deteksi melalui RT-PCR terlalu mahal Uji serologi untuk ToCV [yang jauh lebih murah dari RT-PCR] belum tersedia