Penggunaan CSSLs untuk pencarian lokasi gen dalam kromosom Bogor, 21 Februari 2013
? ? Apakah CSSLs itu? ?
Strategi dasar pemetaan QTL (lokus sifat kuantitatif) Fenotipe: kerapatan trikoma daun Populasi: RILs (recombinant inbred lines) Mauricio, Nature Reviews Genetics, 2001
Metode pemetaan QTL (lokus sifat kuantitatif) Fenotipe: kerapatan trikoma daun Populasi: F2 Mauricio, Nature Reviews Genetics, 2001
Pemetaan awal mencari lokasi Istana Bogor
Pemetaan halus mencari lokasi Istana Bogor
Tahap-tahap berurutan dalam kloning gen-gen QTL Semua tahap ini makan banyak waktu dan tenaga Ashikari & Matsuoka, Trends in Plant Science, 2006
Produksi Introgression Lines (ILs) atau CSSLs Ashikari & Matsuoka, Trends in Plant Science, 2006
Chromosome Segment Substitution Lines (CSSLs) Ebitani et al., Breeding Science , 2005
Pemetaan QTL menggunakan Introgression Lines (ILs) atau CSSLs Paran & Zamir, Trends in Genetics, 2003
Introgression lines of L Introgression lines of L. pennellii in cultivated tomato for QTL mapping FM = fruit mass Left: a fruit from the wild tomato species L. pimpinellifolium Right: a fruit from L. esculentum cv. Giant Red, bred to produce extremely large tomatoes Eshed & Zamir, Genetics, 1995
Figure 2 High-resolution mapping of the fw2.2 QTL. A Frary et al. Science 2000;289:85-88 Published by AAAS
Phenotypic effect of the fw2.2 transgene in L. esculentum pCLD04541 Fruit from R1 progeny of fw107 segregating for the presence (+) or absence (-) of cos50 The exact molecular function of fw2.2 is not known, although it shares homology with the human RAS oncogenes in protein structure and contains two predicted transmembrane-spanning domains. fw2.2 acts as a negative regulator of cell division in the fruit, perhaps via some role in cell-to-cell communication. A Frary et al. Science 2000;289:85-88 Published by AAAS
Positions of QTLs for field resistance to rice blast in Japanese upland rice qBR4-2 146 F4 lines (NipponbarexOwarihatamochi) 111 RFLP markers pi21, a recessive gene encoding a proline-rich protein (Fukuoka et al., Science, 2009) Fukuoka & Okuno, TAG, 2001
Pengembangan bahan tanaman Sub-CSSLs Fukuoka et al., TAG, 2012
Graphical genotypes dari Sub-CSSLs sekitar qBR4-2 Fukuoka et al., TAG, 2012
Graphical genotypes dari Sub-CSSLs sekitar qBR4-2a dan qBR4-2b Fukuoka et al., TAG, 2012
Pyramiding qBR4-2a, qBR4-2b, dan qBR4-2b Fukuoka et al., TAG, 2012
Perbandingan sekuen qBR4-2a antara Nipponbare dan Owarihatamochi Fukuoka et al., TAG, 2012
Peranan protein NBS-LRR dalam ketahanan tanaman terhadap patogen Spoel & Dong, Nat Rev Immunology, 2012
Analisis filogenetik qBR4-2a dan 30 gen R yang lain Fukuoka et al., TAG, 2012
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