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FLOW INJECTION ANALYSIS (Analisis dalam sistem aliran)

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Presentasi berjudul: "FLOW INJECTION ANALYSIS (Analisis dalam sistem aliran)"— Transcript presentasi:

1 FLOW INJECTION ANALYSIS (Analisis dalam sistem aliran)

2 Definition of FIA "Information gathering from a concentration gradient formed from an injected, well-defined zone of a fluid, dispersed into a continuous unsegmented stream of a carrier"  ACADEMIC DEFINITION "a simple and versatile analytical technology for automating wet chemical analysis, based on the physical and chemical manipulation of a dispersed sample zone formed from the injection of the sample into a flowing carrier stream and detection downstream"  INDUSTRIAL DEFINITION "Flow injection analysis should not be explained. It ought to be demonstrated"  Bo Karlberg

3 Working principles of FIA In its simplest form of FIA, the sample zone is injected into a flowing carrier stream of reagent. As the injected zone moves downstream, the sample solution disperses into the reagent, causing the product to form. A flow through detector placed downstream records the desired physical parameter such as colorimetric absorbance or fluorescence.

4 Schematic diagram of FIA

5 Working principles of FIA The typical FIA flow rate is one milliliter per minute; Typical sample volume consumption is 100 microliters per sample; Typical sampling frequency is two samples per minute


7 Main steps of FIA I. Sampling  the sample is measured out and injected into the flowing carrier stream (the name Flow Injection Analysis). This step is generally performed with a sample injection valve. II. Sample processing  To transform the analyte into a species that can be measured by the detector and manipulate its concentration into a range that is compatible with the detector, using one or more of the indicated processes. III. Detection  the analyte, or a derivative of it, generates a signal peak that is used to quantify the compound being determined. As indicated, a large variety of detectors can be used in FIA.

8 Uniqueness of FIA  Kekuatan FIA sebagai alat analisis terletak pada kemampuannya untuk menggabungkan fungsi-fungsi analitis dalam berbagai cara yang berbeda untuk menciptakan berbagai metodologi yang berbeda, dan melaksanakan metodologi ini dengan cepat dan otomatis dengan menit (uL) jumlah sampel.  Tahapan pertama dan terakhir, sebagian besar, teknologi konvensional.  tahap kedua, pengolahan sampel, yang merupakan jantung dari FIA. Sejumlah fungsi yang paling umum pengolahan sampel analitis yang dapat dilakukan oleh FIA digambarkan dalam kotak.  For example, FIA can dilute by factors up to tens of thousands, and can enrich by several hundred.  It can perform chemistry on an analyte to generate a detectable species.  It can transfer an analyte from one medium to another, for example from a gas sample to a FIA carrier, and vice versa.  It can do solvent extraction, and matrix modification or matrix elimination.

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