Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Perhitungan Jumlah Mikrobia Nur Hidayat Kuliah Bio-Industri
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Pembelahan sel tunas Spora Fragmentasi hifa Reproduksi
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Pembelahan Sel Figure 6.11
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Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Jika 100 sel ditumbuhkan selama 5 jam menghasilkan sel maka:
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Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.14
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Jumlah koloni: Dilakukan dengan pengenceran sampel Perhitungan mikrobia berdasar jumlah koloni Figure 6.15, top portion
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Inokulasi cawan petri dari seri pengenceran Metode Plating Figure 6.16
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Setelah inkubasi,hitung koloni pada cewan yang memiliki jumlah koloni (CFU) Penghitungan Koloni Figure 6.15
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Syarat-syarat perhitungan Jumlah koloni 25 – 250. Jika ada dua ulangan dirata-rata, perbandingan dua pengenceran berurutan jika lebih besar atau sama dengan 2 gunakan pengenceran sebelumnya, jika kurang dari 2 dirata-rata. Contoh: PengenceranPetri 1Petri Maka: ( )/( ) = 510/468 < 2 maka Jumlah sel = ( )/4 = 244,5 X10 2 = 2,5 X10 4
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Penggunaan Haemacytometer Figure 6.19
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Perhitungan secara langsung dengan mikroskop Menggunakan haemacytometer atau Petroff Houser Counter. Misal Diket Luas Kotak pada haemacytometer = 4 X mm 2 Tinggi = 2 X mm Maka volume daerah kotak = 8 X mm 3 = 8 X ml Jumlah sel dihitung 14 Maka jumlah mikrobia
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Penghitungan dengan cara tidak langsung Aktivitas metabolit Berat kering
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sampai jumpa